Serum serial number reddit 20212/15/2023 The established method could be used for the rapid detection of the four subgroups of avian metapneumovirus with high specificity and high sensitivity.Ĭitation: Wang S, Jiang N, Jiang L, Zhuang Q, Chen Q, Hou G, et al. Compared with published RT-PCR assays, the κ value of the quadruple real-time RT-PCR assay in 1920 avian clinical samples was 1.000 ( P < 0.001). In the avian clinical sample detection, 39 out of 1920 clinical samples collected from 8 provinces were positive. The other common poultry viruses were negative. The results showed that all the four subgroups of avian metapneumovirus can be detected in the quadruple real-time RT-PCR assay simultaneously, with a detection limit of 100–1000 cRNA copies/reaction. Its specificity and sensitivity were evaluated, and clinical samples were tested by the method. In order to develop an appropriate method for high-throughput detection of avian metapneumovirus, a quadruple real-time reverse-transcription polymerase chain reaction assay was established with four pairs of specific primers and four specific probes based on the G or M gene of aMPV-A, aMPV-B, aMPV-C and aMPV-D.
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